Cell Biolabs, Inc. (CBL) has developed and commercialized innovative technology for Life Science Research. These laboratories provide the best instruments to help define cellular mechanisms, functions, and abnormalities. Their concentration focuses on the development of: Cell-based Assay for adhesion studies, cellular transformation, viral expression and purification, transduction signal products and cytoskeleton regulation.
CytoSelectTM 96-well Cell Transformation Assay (Soft Agar Colony Formation)
Neoplastic transformation occurs via a series of genetic and epigenetic alterations that yield a cell population capable of proliferating independently of both external and internal signals that normally restrain growth. Anchorage-independent growth is one of the hallmarks of transformation, which is considered the most accurate and stringent in vitro assay for detecting malignant transformation of cells. A common method to monitor anchorage-independent growth is using the soft agar colony formation assay, which measures proliferation in a semisolid culture media after 3-4 weeks by manual counting of colonies. This method is quite cumbersome, time-consming, and difficult when testing a large number of samples, The manual counting is also highly subjecting, with varying colony sizes, making it difficult to interpret results.
The CytoSelectTM 96-well Cell Transformation Assay excludes manually counting colonies. Instead cells are incubated 6-8 days in semisolid agar before being solubilized, lysed and detected by the patented CyQuant® GR Dye in a fluorescence plate reader (Figure 1). The CytoSelectTM 96-well Cell Transformation Kit provides a robust system for screening oncogenes and cell transformation inhibitors. Each kit provides sufficient quantities to perform 96 tests in a microtiter plate.
In Addition Cell Biolabs offers cells-based assays for :
- Cell Migration and Invasion
- Adhesion
- Stem Cells
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